Journal
MOLECULES
Volume 26, Issue 4, Pages -Publisher
MDPI
DOI: 10.3390/molecules26041039
Keywords
rutin; solid lipid nanoparticles; Raman spectroscopy; SEM; antioxidant assay; U373 culture cells
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This study successfully prepared and characterized solid lipid nanoparticles loading rutin, showing potential therapeutic effects in treating oxidative stress-induced diseases. Through various analyses, it was found that solid lipid nanoparticles efficiently encapsulated rutin, with excellent release properties and potential antioxidant activity.
This study was aimed at preparing and characterizing solid lipid nanoparticles loading rutin (RT-SLNs) for the treatment of oxidative stress-induced diseases. Phospholipon 80H(R) as a solid lipid and Polysorbate 80 as surfactant were used for the SLNs preparation, using the solvent emulsification/diffusion method. We obtained spherical RT-SLNs with low sizes, ranging from 40 to 60 nm (hydrodynamic radius) for the SLNs prepared starting from 2% and 5% (w/w) theoretical amount. All prepared formulations showed negative zeta-potential values. RT was efficiently encapsulated within SLNs, obtaining high encapsulation efficiency and drug content percentages, particularly for SLNs prepared with a 5% theoretical amount of RT. In vitro release profiles and analysis of the obtained data applying different kinetic models revealed Fickian diffusion as the main mechanism of RT release from the SLNs. The morphology of RT-SLNs was characterized by scanning electron microscopy (SEM), whereas the interactions between RT and the lipid matrix were investigated by Raman spectroscopy, evidencing spectral modifications of characteristic bands of RT due to the establishment of new interactions. Finally, antioxidant activity assay on human glioblastoma astrocytoma (U373) culture cells showed a dose-dependent activity for RT-SLNs, particularly at the highest assayed dose (50 mu M), whereas the free drug showed the lesser activity.
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