Overview of Popular Techniques of Raman Spectroscopy and Their Potential in the Study of Plant Tissues

Raman spectroscopy is one of the main analytical techniques used in optical metrology. It is a vibration, marker-free technique that provides insight into the structure and composition of tissues and cells at the molecular level. Raman spectroscopy is an outstanding material identification technique. It provides spatial information of vibrations from complex biological samples which renders it a very accurate tool for the analysis of highly complex plant tissues. Raman spectra can be used as a fingerprint tool for a very wide range of compounds. Raman spectroscopy enables all the polymers that build the cell walls of plants to be tracked simultaneously; it facilitates the analysis of both the molecular composition and the molecular structure of cell walls. Due to its high sensitivity to even minute structural changes, this method is used for comparative tests. The introduction of new and improved Raman techniques by scientists as well as the constant technological development of the apparatus has resulted in an increased importance of Raman spectroscopy in the discovery and defining of tissues and the processes taking place in them.

Automated summary

Raman spectroscopy is a vibration, marker-free technique used for analyzing tissue and cell structure and composition at the molecular level, providing accurate material identification and serving as a fingerprint tool for a wide range of compounds. The sensitivity of this method to even minute structural changes allows for comparative tests and facilitates tracking of polymers in plant cell walls, contributing to an increased importance in tissue discovery and definition thanks to advancements in both techniques and technology.