Journal
MOLECULES
Volume 26, Issue 6, Pages -Publisher
MDPI
DOI: 10.3390/molecules26061514
Keywords
styrene monooxygenase; whole-cell biocatalysis; chiral epoxides
Funding
- Slovak Research and Development Agency [PP-COVID-20-0056]
- Slovak Grant Agency for Science VEGA [1/0552/18]
- Operational Program Integrated Infrastructure - European Regional Development Fund [313021X329]
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Styrene monooxygenases are highly selective enzymes that can catalyze the epoxidation of alkenes with high enantioselectivity. The expression of two-component recombinant styrene monooxygenase in E. coli allows for the efficient preparation of chiral compounds containing oxirane rings.
Styrene monooxygenases are a group of highly selective enzymes able to catalyse the epoxidation of alkenes to corresponding chiral epoxides in excellent enantiopurity. Chiral compounds containing oxirane ring or products of their hydrolysis represent key building blocks and precursors in organic synthesis in the pharmaceutical industry, and many of them are produced on an industrial scale. Two-component recombinant styrene monooxygenase (SMO) from Marinobacterium litorale was expressed as a fused protein (StyAL2StyB) in Escherichia coli BL21(DE3). By high cell density fermentation, 35 g(DCW)/L of biomass with overexpressed SMO was produced. SMO exhibited excellent stability, broad substrate specificity, and enantioselectivity, as it remained active for months and converted a group of alkenes to corresponding chiral epoxides in high enantiomeric excess (>95-99% ee). Optically pure (S)-4-chlorostyrene oxide, (S)-allylbenzene oxide, (2R,5R)-1,2:5,6-diepoxyhexane, 2-(3-bromopropyl)oxirane, and (S)-4-(oxiran-2-yl)butan-1-ol were prepared by whole-cell SMO.
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