4.6 Article

Folate Content and Yolk Color of Hen Eggs from Different Farming Systems

Journal

MOLECULES
Volume 26, Issue 4, Pages -

Publisher

MDPI
DOI: 10.3390/molecules26041034

Keywords

folates; vitamins; food folate analysis; organic food; functional foods; HPLC; color analysis; yolk color

Funding

  1. program entitled Regional Initiative of Excellence for the years 2019-2022 [010/RID/2018/19]
  2. University of Warmia and Mazury [17.610.008-300]

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The study compared folate contents in hen eggs from different farming systems, with organic eggs having the highest folate content. One egg may provide 40-86 μg of folate, which is 10-22% of the recommended daily intake for adults. The most stable form of folate found in eggs was 5-methyltetrahydrofolate.
This study aimed to compare folate contents in hen eggs from four different farming systems, namely organic, free range, barn, and cage one. Folate retention during egg boiling was studied as well. The contents of individual folate vitamers were determined using the high-performance liquid chromatography method (HPLC), following trienzyme treatment. Folate content in eggs differed significantly (p < 0.05) due to the rearing system, with the highest mean content determined in the eggs from organic farming (113.8 mu g/100 g). According to this study, one egg (60 g) may provide 40-86 mu g of folates, which corresponds to 10-22% of the recommended daily intake for adults, 400 mu g according to the Nutrition Standards for the Polish Population. The predominant folate form found in egg was 5-methyltetrahydrofolate, which showed considerably greater stability under boiling compared to 10-formylfolic acid present in a lower amount. In most eggs tested, the losses in total folate content did not exceed 15%. The color of yolk of the most folate-abundant organic eggs, had the highest value of lightness (L*) and the lowest value of redness (a*). This, however, does not correspond to consumer preferences of intense golden yolk color.

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