Journal
MOLECULAR PHARMACEUTICS
Volume 18, Issue 3, Pages 1238-1246Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.molpharmaceut.0c01107
Keywords
near-infrared photoimmunotherapy; optical imaging; IR700; near-infrared camera; indocyanine green
Funding
- Intramural Research Program of the National Institutes of Health, National Cancer Institute
- Center for Cancer Research [ZIABC011513]
- National Center for Global Health and Medicine Research Institute, Tokyo, Japan
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Near-infrared photoimmunotherapy (NIR-PIT) is a newly developed cancer treatment that utilizes specific wavelength near-infrared light to target tumor cells for treatment. Research has shown that conjugation of a second near-infrared dye prior to NIR-PIT can provide real-time feedback on tumor location.
Near-infrared photoimmunotherapy (NIR-PIT) is a newly developed cancer treatment that uses antibody-IRDye700DX (IR700) conjugates and was recently approved in Japan for patients with inoperable head and neck cancer. Exposure of the tumor with NIR light at a wavelength of 690 nm leads to physicochemical changes in the antibody-IR700 conjugate-cell receptor complex, resulting in increased hydrophobicity and damage to the integrity of the cell membrane. However, it is important that the tumor be completely exposed to light during NIR-PIT, and thus, a method to provide real-time information on tumor location would help clinicians direct light more accurately. IR700 is a fluorophore that emits at 702 nm; however, there is no clinically available device optimized for detecting this fluorescence. On the other hand, many indocyanine green (ICG) fluorescence imaging devices have been approved for clinical use in operating rooms. Therefore, we investigated whether LIGHTVISION, one of the clinically available ICG cameras, could be employed for NIR-PIT target tumor detection. Due to the limited benefits of adding IR700 molecules, the additional conjugation of IRDye800CW (IR800) or ICG-EG4-Sulfo-OSu (ICG-EG4), which has an overlapping spectrum with ICG, to trastuzumab-IR700 conjugates was performed. Conjugation of second NIR dyes did not interfere the efficacy of NIR-PIT. The dual conjugation of IR800 and IR700 to trastuzumab clearly visualized target tumors with LIGHTVISION by detecting emission light of IR800. We demonstrated that the conjugation of second NIR dyes enables us to provide a real-time feedback of tumor locations prior to NIR-PIT.
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