Journal
MOLECULAR CELL
Volume 81, Issue 7, Pages 1515-+Publisher
CELL PRESS
DOI: 10.1016/j.molcel.2021.01.019
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Funding
- Cancer Prevention and Research Institute [RP200254]
- NIH [5P01CA092584-19]
- CPRIT [RP170628]
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Loss of ATM kinase leads to neurodegeneration and protein aggregation, driven by PARPs associating with PAR-associated genomic sites, causing protein species to arise from intrinsically disordered proteins. Single-strand DNA breaks, dependent on reactive oxygen species, transcription, and R-loops, are implicated in this process. The phenomenon is also observed in Mre11 A-T-like disorder mutants, providing a hypothesis for protein integrity loss and cerebellum dysfunction in A-T.
Loss of the ataxia-telangiectasia mutated (ATM) kinase causes cerebellum-specific neurodegeneration in humans. We previously demonstrated that deficiency in ATM activation via oxidative stress generates insoluble protein aggregates in human cells, reminiscent of protein dysfunction in common neurodegenerative disorders. Here, we show that this process is driven by poly-ADP-ribose polymerases (PARPs) and that the insoluble protein species arise from intrinsically disordered proteins associating with PAR-associated genomic sites in ATM-deficient cells. The lesions implicated in this process are single-strand DNA breaks dependent on reactive oxygen species, transcription, and R-loops. Human cells expressing Mre11 A-T-like disorder mutants also show PARP-dependent aggregation identical to ATM deficiency. Lastly, analysis of A-T patient cerebellum samples shows widespread protein aggregation as well as loss of proteins known to be critical in human spinocerebellar ataxias that is not observed in neocortex tissues. These results provide a hypothesis accounting for loss of protein integrity and cerebellum function in A-T.
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