In situ controllable synthesis of Schiff base networks porous polymer coatings for open-tubular capillary electrochromatography

A uniform Schiff base network (SNW) film was synthesized in situ in a controllable way through continuous flow of reactants inside the capillary. The properties and application of the as-prepared capillary was investigated in capillary electrochromatography. The effects of reaction monomer concentration and reaction time on coating thickness were studied by SEM. The results show that the reaction condition has a significant influence on the morphology and thickness of the SNW films. The thickness of the film can be controlled by changing the concentration of reaction solution and reaction time. Capillaries coated under different conditions were employed to separate four nucleotides by capillary electrochromatography, which demonstrated significant variation of migration time, peak order, and separation efficiency. Analytes containing nitrogen heterocycle structures, such as nucleotides, methylimidazole isomers, and beta-lactam antibiotics, were successfully separated with the prepared open-tubular columns. Under the selected separation conditions, theoretical plate number of four nucleotides is in a range 45,237-104,505 plates.m(-1), and the resolutions are 1.98-8.07. A resolution of 1.75 is obtained for methylimidazole isomers. The nucleotides in a real sample, chicken essence seasoning, were determined using the prepared capillary column with satisfactory recoveries in the range 95 to 105%.

Automated summary

A uniform Schiff base network film was synthesized in a controllable way by continuously flowing reactants inside a capillary. The research investigated the effects of reaction conditions on the morphology and thickness of the film, showing that thickness can be controlled by altering reaction concentration and time. The capillaries under different conditions were used for capillary electrochromatography, resulting in significant variations in migration time, peak order, and separation efficiency, particularly for analytes with nitrogen heterocycle structures.