Journal
MICROBIOLOGICAL RESEARCH
Volume 243, Issue -, Pages -Publisher
ELSEVIER GMBH
DOI: 10.1016/j.micres.2020.126649
Keywords
Nonphotosynthetic algae; Polytomella parva; Chlorophycean algae; Chlamydomonas reinhardtii; Amyloplast; Plastid proteome
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Funding
- Consejo Nacional de Ciencia y Tecnologia, CONACyT, Mexico [239219, 279125]
- Direccion General de Asuntos del Personal Academico (DGAPA-UNAM, Mexico) [IN209220]
- CONACyT [261684]
- Sistema Nacional de Investigadores (Mexico)
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This study provides a morphological and proteomic characterization of the colorless alga Polytomella parva, identifying several plastid proteins involved in various metabolic pathways. The results contribute to a better understanding of the metabolism and biological activities of this unicellular, free-living alga.
The unicellular, free-living, nonphotosynthetic chlorophycean alga Polytomella parva, closely related to Chlamydomonas reinhardtii and Volvox carteri, contains colorless, starch-storing plastids. The P. parva plastids lack all light-dependent processes but maintain crucial metabolic pathways. The colorless alga also lacks a plastid genome, meaning no transcription or translation should occur inside the organelle. Here, using an algal fraction enriched in plastids as well as publicly available transcriptome data, we provide a morphological and proteomic characterization of the P. parva plastid, ultimately identifying several plastid proteins, both by mass spectrometry and bioinformatic analyses. Data are available via ProteomeXchange with identifier PXD022051. Altogether these results led us to propose a plastid proteome for P. parva, i.e., a set of proteins that participate in carbohydrate metabolism; in the synthesis and degradation of starch, amino acids and lipids; in the biosynthesis of terpenoids and tetrapyrroles; in solute transport and protein translocation; and in redox homeostasis. This is the first detailed plastid proteome from a unicellular, free-living colorless alga.
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