Journal
METHODS
Volume 203, Issue -, Pages 276-281Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2021.02.018
Keywords
CRISPR; CRISPR diagnostics; Cas12; Trans-cleavage; HOLMES
Funding
- National Natural Science Foundation of China [31922046]
- Sanming Project of Medicine in Shenzhen [SZSM202011017]
- Commissioned Health and Medical Research Fund of Food and Health Bureau of HKSAR Government [COVID190102]
- Special Program for Technical Support of the State Administration for Market Regulation [2019YJ018]
- UAEU [31R0135, 31R246]
- Al Jalila Foundation [21M132]
- Scientific Research Foundation of Taizhou [2001xg07]
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This review mainly focuses on the applications of Cas12-based methods in nucleic acids detection and beyond. CRISPR has become the most popular technology worldwide due to its outstanding contribution in genome editing, and the discovery of nonspecific trans-cleavage activities of Cas proteins such as Cas12 provides advantages for the successful creation of CRISPR-based molecular diagnostic systems.
Because of the outstanding contribution in genome editing, CRISPR has undoubtedly become the most popular technology around the world and two pioneers are awarded the Nobel Prize in Chemistry this year. Besides, along with the discovery of nonspecific trans-cleavage activities of several Cas proteins such as Cas12 and Cas13, many CRISPR-based molecular diagnostic systems have been successfully created, showing advantages in sensitivity, specificity and operation convenience. Among them, systems with Cas12, which targets DNA and trans-cleaves single-stranded DNA probes, are both simple and highly efficient. Here in this review, we mainly focus on the Cas12-based methods and briefly discuss their applications in nucleic acids detection and beyond.
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