Journal
LWT-FOOD SCIENCE AND TECHNOLOGY
Volume 139, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.lwt.2020.110605
Keywords
Whey protein isolate; Zein; Complex nanoparticle; pH-shifting method; Environmental stability
Categories
Funding
- National Natural Science Foundation of China [31871842]
Ask authors/readers for more resources
The alcohol-free pH-shifting method was used to fabricate stable complex nanoparticles from hydrophilic whey protein isolate (WPI) and hydrophobic zein, showing desirable storage and physical stability, as well as excellent stability against various environmental stresses. The formation of WPI-zein complex nanoparticles was influenced by hydrophobic effects and electrostatic attraction, with a core-shell structure observed in transmission electron microscope (TEM) images. This study demonstrates the feasibility of using the pH-shifting method to fabricate protein-based complex nanoparticles for delivery of bioactive compounds.
The alcohol-free pH-shifting method was applied to fabricate stable complex nanoparticles from hydrophilic whey protein isolate (WPI) and hydrophobic zein, which was aimed to develop a strategy to obtain desirable physicochemical stability. When the mass ratio of WPI and zein was elevated from 9:1 to 1:1, the size of WPI-zein complex nanoparticles was increased from 90 to 150 nm. The complex nanoparticle showed desirable storage stability (28 days) and physical stability, as well as excellent stability against various environmental stresses (heat, pH and ionic strength). The analysis of FTIR and the nanoparticle dissociation test revealed that hydrophobic effects and electrostatic attraction played an important role in the formation of WPI-zein complex nanoparticles. The transmission electron microscope (TEM) images evidenced the core-shell structure of WPI-zein complex nanoparticles with a regular spherical shape. The findings showed the feasibility of the simple pH-shifting method to fabricate the protein-based complex nanoparticles, which were expected to be further applied in the delivery of bioactive compounds.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available