Journal
LWT-FOOD SCIENCE AND TECHNOLOGY
Volume 138, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.lwt.2020.110683
Keywords
Zea mays L.; Biofortification; Mass spectrometry; Amino acids
Categories
Funding
- Fundacno de Amparo a Pesquisa do Estado de Sao Paulo [12/02623-2, 19/18346-7]
- Brazilian Biosciences National Laboratory (LNBio) [MAS-16477, MAS-19440]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [12/02623-2, 19/18346-7] Funding Source: FAPESP
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Biofortification has led to the development of quality protein maize (QPM) with essential amino acids. Study comparing QPM cultivars with a non-QPM hybrid in Brazil showed compositional equivalence with differential expression of proteins, indicating superior nutritional value and susceptibility to pathogens.
Biofortification of crops has promoted the development of quality protein maize (QPM), which are rich in essential amino acids. However, the proteomic rebalancing of QPM cultivars grown in Brazil remains unknown. Here, we show the compositional equivalence and protein profiles from two QPM cultivars (BR-473 and BR-451, named here as QPM1 and QPM2, respectively) compared to a commercial non-QPM hybrid (BRS-4103, non-QPM), by using complementary 2-DE-based (gel-based) and bottom-up shotgun (gel-free) proteomic approaches. The compositional equivalence observed in these cultivars was expected for Zea mays. Among differentially expressed proteins (DEPs) identified, storage proteins as non-zeins were generally up-regulated, where zeins were down-regulated in QPM cultivars. In turn, defense and cell stress proteins as heat shock protein (HSP) and ribosome-inactivating protein (RIP) were down-regulated. Together, these findings point the superior nutritional value of proteins from QPM cultivars as well as their susceptibility to pathogens.
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