Journal
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES
Volume 76, Issue 12, Pages 2112-2121Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/gerona/glab077
Keywords
Aging; Circadian Rhythm; Disuse; Ribosome profiling; Translation
Categories
Funding
- Ruth L. Kirschstein National Research Service Award [R21AR073422, NIH 1T32HL139451]
- National Center for Advancing Translational Sciences of the National Institutes of Health [UL1TR002538]
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Periods of inactivity in older adults lead to resistance to nutrient metabolism, causing abnormalities in skeletal muscle transcription and translation, which contributes to muscle dysfunction. Leucine can alter the translation of genes related to inactivity, as well as increase the translational efficiency of certain transcripts independent of mRNA abundance.
Periods of inactivity experienced by older adults induce nutrient anabolic resistance creating a cascade of skeletal muscle transcriptional and translational aberrations contributing to muscle dysfunction. The purpose of this study was to identify how inactivity alters leucine-stimulated translation of molecules and pathways within the skeletal muscle of older adults. We performed ribosomal profiling alongside RNA sequencing from skeletal muscle biopsies taken from older adults (n = 8; similar to 72 years; 6 F/2 M) in response to a leucine bolus before (Active) and after (Reduced Activity) 2 weeks of reduced physical activity. At both visits, muscle biopsies were taken at baseline, 60 minutes (early response), and 180 minutes (late response) after leucine ingestion. Previously identified inactivity-related gene transcription changes (PFKFB3, GADD45A, NMRK2) were heightened by leucine with corresponding changes in translation. In contrast, leucine also stimulated translational efficiency of several transcripts in a manner not explained by corresponding changes in mRNA abundance (uncoupled translation). Inactivity eliminated this uncoupled translational response for several transcripts, and reduced the translation of most mRNAs encoding for ribosomal proteins. Ingenuity Pathway Analysis identified discordant circadian translation and transcription as a result of inactivity such as translation changes to PER2 and PER3 despite unchanged transcription. We demonstrate inactivity alters leucine-stimulated uncoupled translation of ribosomal proteins and circadian regulators otherwise not detectable by traditional RNA sequencing. Innovative techniques such as ribosomal profiling continues to further our understanding of how physical activity mediates translational regulation, and will set a path toward therapies that can restore optimal protein synthesis on the transcript-specific level to combat negative consequences of inactivity on aging muscle.
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