A Genetic Map and Linkage Panel for the Large-fruited Fresh-market Tomato

Genetic maps saturated with genetic markers are useful for genetic research and crop breeding; however, the genetic map for the large-fruited fresh-market tomato (Solanum lycopersicum) has never been constructed, and the recombination frequency between DNA fragments is only partly understood for fresh-market tomato. We constructed a novel fresh-market tomato genetic map by using 3614 single nucleotide polymorphism (SNP) markers and a 93 F-2 segregating progeny derived from a cross between two United States large-fruited fresh-market tomato lines. The average distance between markers was less than 1 cM, and substantial recombination densities between markers were observed across the approximate centromere locations. A linkage panel for large-fruited fresh-market tomato was also established using the combined dataset of the genetic map and 58 SNP-genotyped core tomato lines. The allelic information in the linkage panel will be a significant resource for both tomato genetics and future breeding approaches.

Automated summary

A novel genetic map for large-fruited fresh-market tomato was constructed using 3614 SNP markers and a segregating progeny, revealing substantial recombination densities and linkage relationships. Additionally, a linkage panel was established for large-fruited fresh-market tomato, providing valuable allelic information for tomato genetics and future breeding strategies.