Journal
JOURNAL OF STRUCTURAL BIOLOGY
Volume 213, Issue 1, Pages -Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jsb.2020.107695
Keywords
Electron microscopy; Single particle analysis; Preferred orientations
Funding
- la Caixa Foundation [LCF/BQ/DI18/11660021]
- European Union [713673]
- Spanish Ministry of Economy and Competitiveness [BIO2016-76400-R, SEV 2017-0712]
- Comunidad Autonoma de Madrid [S2017/BMD-3817]
- Instituto de Salud Carlos III [PT17/0009/0010 (ISCIII-SGEFI/ERDF)]
- European Union (EU) [654248, 731005, 824087, 810057, 857203, 857647]
- European Research Council (ERC) [810057] Funding Source: European Research Council (ERC)
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The presence of preferred orientations in SPA by cryoEM poses a challenge for achieving high-resolution structures, but some image processing algorithms are more robust against these detrimental conditions.
The presence of preferred orientations in single particle analysis (SPA) by cryo-Electron Microscopy (cryoEM) is currently one of the hurdles preventing many structural analyses from yielding high-resolution structures. Although the existence of preferred orientations is mostly related to the grid preparation, in this technical note, we show that some image processing algorithms used for angular assignment and three-dimensional (3D) reconstruction are more robust than others to these detrimental conditions. We exemplify this argument with three different data sets in which the presence of preferred orientations hindered achieving a 3D reconstruction without artifacts or, even worse, a 3D reconstruction could never be achieved.
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