4.7 Article

Cross-Species Proteomic Comparison of Outer Membrane Vesicles and Membranes of Francisella tularensis subsp. tularensis versus subsp. holarctica

Journal

JOURNAL OF PROTEOME RESEARCH
Volume 20, Issue 3, Pages 1716-1732

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/acs.jproteome.0c00917

Keywords

Francisella tularensis; FSC200; SchuS4; cross-species proteomic comparison; outer membrane vesicles; virulence factor

Funding

  1. Grant Agency of the Czech Republic [17-04010S]
  2. Ministry of Defense of the Czech Republic (Long-term organization development plan Medical Aspects of Weapons of Mass Destruction)

Ask authors/readers for more resources

The study compares the proteomic composition of OMV and membrane fractions from two strains of Francisella tularensis, proposing a novel approach for cross-species proteomic comparison. By consecutively comparing the subproteomes of the two strains, differences in protein amounts caused by global expression changes can be distinguished from those caused by preferential protein packing into OMV or membranes.
Release of outer membrane vesicles (OMV) is an important phenomenon in Gram-negative bacteria playing multiple roles in their lifestyle, including in relation to virulence and host-pathogen interaction. Francisella tularensis, unlike other bacteria, releases unusually shaped, tubular OMV. We present a proteomic comparison of OMV and membrane fractions from two F. tularensis strains: moderately virulent subsp. holarctica strain FSC200 and highly virulent subsp. tularensis strain SchuS4. Proteomic comparison studies routinely evaluate samples from the same proteome, but sometimes we must compare samples from closely related organisms. This raises quantification issues. We propose a novel approach to cross-species proteomic comparison based on an intersection protein database from the individual single-species databases. This is less prone to quantification errors arising from differences in the sequences. Consecutively comparing subproteomes of OMV and membranes of the two strains allows distinguishing differences in relative protein amounts caused by global expression changes from those caused by preferential protein packing to OMV or membranes. Among the proteins most differently packed into OMV between the two strains, we detected proteins involved in biosynthesis and metabolism of bacterial envelope components like O-antigen, lipid A, phospholipids, and fatty acids, as well as some major structural outer membrane proteins. The data are available via ProteomeXchange with identifier PXD022406.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available