Journal
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
Volume 12, Issue 8, Pages 2166-2171Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jpclett.0c03570
Keywords
-
Categories
Funding
- NSF-RAPID Grant [DMR-2030439]
- NSF-PREM Grant [DMR-1826886]
- NIH-NIMHD Grant [1U54MD015929-01]
- NASA award [80NSSC19K1603]
- COVID19 funds
Ask authors/readers for more resources
In this study, a rapid diagnostic method for specific SARS-CoV-2 antigens and pseudotyped viruses was demonstrated using Rhodamine 6G dye conjugated DNA aptamer-attached gold nanostars. Additionally, it was shown that DNA aptamer-attached GNSs can prevent virus infection by inhibiting receptor binding and destroying the virus membrane.
The ongoing outbreak of the coronavirus infection has killed more than 2 million people. Herein, we demonstrate that Rhodamine 6G (Rh-6G) dye conjugated DNA aptamer-attached gold nanostar (GNS)-based distance-dependent nanoparticle surface energy transfer (NSET) spectroscopy has the capability of rapid diagnosis of specific SARS-CoV-2 spike recombinant antigen or SARS-CoV-2 spike protein pseudotyped baculovirus within 10 min. Because Rh-6G-attached single-stand DNA aptamer wrapped the GNS, 99% dye fluorescence was quenched because of the NSET process. In the presence of spike antigen or virus, the fluorescence signal persists because of the aptamer-spike protein binding. Specifically, the limit of detection for the NSET assay has been determined to be 130 fg/mL for antigen and 8 particles/mL for virus. Finally, we have demonstrated that DNA aptamer-attached GNSs can stop virus infection by blocking the angiotensin-converting enzyme 2 (ACE2) receptor binding capability and destroying the lipid membrane of the virus.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available