Journal
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Volume 195, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.jpba.2020.113835
Keywords
Streptomyces sp.; Actinomycin D; Actinomycin X-2; Supercritical fluid chromatography
Categories
Funding
- National Key Research and Development Project [2018YFA0902504, 2019YFC0312501]
- Science and Technology Project of Shenzhen City, Shenzhen Bureau of Science, Technology, and Information [JCYJ20180305123659726, JCYJ20180507182405562]
- Innovation -Driven Development Special Fund Project of Guangxi [Guike AA18242047]
- Grant Plan for Demonstration Project for Marine Economic Development in Shenzhen [86]
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This study successfully utilized supercritical fluid chromatography (SFC) for the purification of Actinomycin X-2 and actinomycin D from a marine derived Streptomyces sp. DQS-5, providing a green alternative for their separation. The study also demonstrated the development of a simple and rapid method for the separation of natural products based on SFC.
Actinomycin D has long been used as a first-line antitumor drug in clinical practice. Actinomycin X-2, a new drug lead, is often isolated along with actinomycin D. The minor differences between the two actinomycin analogs pose a daunting challenge in separation. In this study, supercritical fluid chromatography (SFC) was successfully utilized for the purification of actinomycin X-2 and actinomycin D from a marine derived Streptomyces sp. DQS-5. After one-step SFC purification, the purities of these two compounds were determined to be 97.3 % and 97.8 %, respectively. This method provides a green alternative for the separation of these pharmacologically important actinomycin antibiotics. This study also demonstrated the development of a simple and rapid method for the separation of natural products based on SFC. (C) 2020 Elsevier B.V. All rights reserved.
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