4.6 Article

Efficient, green, and rapid strategy for separating actinomycin D and X2 using supercritical fluid chromatography

Journal

Publisher

ELSEVIER
DOI: 10.1016/j.jpba.2020.113835

Keywords

Streptomyces sp.; Actinomycin D; Actinomycin X-2; Supercritical fluid chromatography

Funding

  1. National Key Research and Development Project [2018YFA0902504, 2019YFC0312501]
  2. Science and Technology Project of Shenzhen City, Shenzhen Bureau of Science, Technology, and Information [JCYJ20180305123659726, JCYJ20180507182405562]
  3. Innovation -Driven Development Special Fund Project of Guangxi [Guike AA18242047]
  4. Grant Plan for Demonstration Project for Marine Economic Development in Shenzhen [86]

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This study successfully utilized supercritical fluid chromatography (SFC) for the purification of Actinomycin X-2 and actinomycin D from a marine derived Streptomyces sp. DQS-5, providing a green alternative for their separation. The study also demonstrated the development of a simple and rapid method for the separation of natural products based on SFC.
Actinomycin D has long been used as a first-line antitumor drug in clinical practice. Actinomycin X-2, a new drug lead, is often isolated along with actinomycin D. The minor differences between the two actinomycin analogs pose a daunting challenge in separation. In this study, supercritical fluid chromatography (SFC) was successfully utilized for the purification of actinomycin X-2 and actinomycin D from a marine derived Streptomyces sp. DQS-5. After one-step SFC purification, the purities of these two compounds were determined to be 97.3 % and 97.8 %, respectively. This method provides a green alternative for the separation of these pharmacologically important actinomycin antibiotics. This study also demonstrated the development of a simple and rapid method for the separation of natural products based on SFC. (C) 2020 Elsevier B.V. All rights reserved.

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