Rod Photoreceptors Avoid Saturation in Bright Light by the Movement of the G Protein Transducin

Rod photoreceptors can be saturated by exposure to bright background light, so that no flash superimposed on the background can elicit a detectable response. This phenomenon, called increment saturation, was first demonstrated psychophysically by Aguilar and Stiles and has since been shown in many studies to occur in single rods. Recent experiments indicate, however, that rods may be able to avoid saturation under some conditions of illumination. We now show in ex vivo electroretinogram and single-cell recordings that in continuous and prolonged exposure even to very bright light, the rods of mice from both sexes recover as much as 15% of their dark current and that responses can persist for hours. In parallel to recovery of outer segment current is an ;10-fold increase in the sensitivity of rod photoresponses. This recovery is decreased in transgenic mice with reduced light-dependent translocation of the G protein transducin. The reduction in outer-segment transducin together with a novel mechanism of visual-pigment regeneration within the rod itself enable rods to remain responsive over the whole of the physiological range of vision. In this way, rods are able to avoid an extended period of transduction channel closure, which is known to cause photoreceptor degeneration.

Automated summary

Rod photoreceptors can become saturated by bright background light, but recent experiments have shown that they can also recover sensitivity and maintain responses even under prolonged exposure to bright light. The reduction of translocation of G protein transducin in transgenic mice may affect the recovery ability, and a novel mechanism of visual-pigment regeneration within the rod itself enables them to remain responsive. This allows rods to avoid extended closure of transduction channels, which could lead to photoreceptor degeneration.