New AlIIIZnII and AlIIICuII dinuclear complexes: Phosphatase-like activity and cytotoxicity

Herein, we report the synthesis and characterization of the first two Al-III(mu-OH)M-II (M = Zn (1) and Cu (2)) complexes with the unsymmetrical ligand H2L-{2-[[(2-hydroxybenzyl)(2-pyridylmethyelaminomethyl]-6-bis (pyridylmethyl)aminomethyl}-4-methylphenol. The complexes were characterized through elemental analysis, X-ray crystallography, IR spectroscopy, mass spectrometry and potentiometric titration. In addition, complex 2 was characterized by electronic spectroscopy. Kinetics studies on the hydrolysis of the model substrate bis(2,4-dinitrophenyl)phosphate by 1 and 2 show Michaelis-Menten behavior, with 1 being slightly more active (8.31%) than 2 (at pH 7.0). The antimicrobial effect of the compounds was studied using four bacterial strains (Staphylococcus aureus, Pseudomonas aeuruginosa, Shigella sonnei and Shigella dysenteriae) and for both complexes the inhibition of bacterial growth was superior to that caused by sulfapyridine, but inferior to that of tetracycline. The dark cytotoxicity and photocytotoxicity (under UV-A light) of the complexes in a chronic myelogenous leukemia cell line were investigated. Complexes 1 and 2 exhibited significant cytotoxic activity against K562 cells, which undergoes a 2-fold increase on applying 5 min of irradiation with UV-A light. Complex 2 was more effective and a good correlation between cytotoxicity and intracellular concentration was observed, the intracellular copper concentration required to inhibit 50% of cell growth being 3.5 x 10(-15) mol cell(-1).

Automated summary

In this study, two novel Al-III-Zn and Al-III-Cu complexes were synthesized and characterized, showing good antimicrobial activity against bacterial strains. These complexes also exhibited cytotoxicity against chronic myelogenous leukemia cells, suggesting potential anti-tumor activity.