Journal
JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY
Volume 96, Issue 4, Pages 469-478Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/14620316.2021.1882887
Keywords
Pyrus; vacuolar processing enzymes; sequence analysis; enzyme activity; eukaryotic expression
Categories
Funding
- Fundamental Research Funds for the Central Universities in China [KYZ201834]
Ask authors/readers for more resources
This study identified eight members of the VPE family in pear genome, with PbrVPE2 and PbrVPE5 showing abundant expression during pear fruit development and ripening. Their biological function was predicted through recombinant protein expression and subcellular localization.
Vacuolar processing enzymes (VPEs) are important cysteine proteases and function in the processing and maturation of protein precursors, plant senescence and immunity, programmed cell death, as well as sugar accumulation. This paper investigated VPEs in pear and identified as a key finding eight members of the VPE family in the pear (Pyrus) genome, and their gene structure, protein conserved domains and phylogenetic relationship were analysed by bioinformatics methods. Further key findings were that two of these VPEs (PbrVPE2 and PbrVPE5) represented a vegetative and the others a seed-coat type VPE. Transcriptome data and quantitative real-time PCR were used separately to analyse the expression patterns of the pear VPE gene family. The PbrVPE2 and PbrVPE5 were, in comparison to the other VPEs, abundantly expressed by possibly playing a role during the development and ripening of the pear fruit. A highly expressed PbrVPE2 gene was isolated from a pear fruit and named PpyVPE2. The biological function of the newly PpyVPE2 genes was predicted by recombinant protein expression and subcellular localisation.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available