Prediction of donor related lung injury in clinical lung transplantation using a validated ex vivo lung perfusion inflammation score

BACKGROUND: Ex vivo lung perfusion (EVLP) is an isolated organ assessment technique that has revo-lutionized the field of lung transplantation and enabled a safe increase in the number of organs trans-planted. The objective of this study was to develop a protein-based assay that would provide a precision medicine approach to lung injury assessment during EVLP. METHODS: Perfusate samples collected from clinical EVLP cases performed from 2009 to 2019 were separated into development (n = 281) and validation (n = 57) sets to derive and validate an inflamma-tion score based on IL-6 and IL-8 protein levels in perfusate. The ability of an inflammation score to predict lungs suitable for transplantation and likely to produce excellent recipient outcomes (time on ventilator <= 3 days) was assessed. Inflammation scores were compared to conventional clinical EVLP assessment parameters and associated with outcomes, including primary graft dysfunction and patient care in the ICU. RESULTS: An inflammation score accurately predicted the decision to transplant (AUROC 68% [95% CI 62-74]) at the end of EVLP and those transplants associated with short ventilator times (AUROC 73% [95% CI 66-80]). The score identified lungs more likely to develop primary graft dysfunction at 72-hours post-transplant (OR 4.0, p = 0.03). A model comprised of the inflammation score and Delta PO2 was able to determine EVLP transplants that were likely to have excellent recipient outcomes, with an accuracy of 87% [95% CI 83-92]. CONCLUSIONS: The adoption of an inflammation score will improve accuracy of EVLP decision-mak-ing and increase confidence of surgical teams to determine lungs that are suitable for transplantation, thereby improving organ utilization rates and patient outcomes. (C) Published by Elsevier Inc. on behalf of International Society for Heart and Lung Transplantation.

Automated summary

This study developed a protein-based assay using IL-6 and IL-8 levels in perfusate to assess lung injury during EVLP, accurately predicting transplant suitability and recipient outcomes. The inflammation score in conjunction with Delta PO2 successfully identified EVLP transplants likely to have favorable recipient outcomes, improving organ utilization rates and patient outcomes.