Journal
JOURNAL OF HAZARDOUS MATERIALS
Volume 415, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.jhazmat.2021.125647
Keywords
Yeast; Tobacco plant; sequestration; Phytoremediation; Food safety; Efflux and translocation
Categories
Funding
- Jiangsu Provincial Natural Science Foundation of China [BK20200093]
- National Natural Science Foundation of China [42077122, 21637002, 21707068]
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The ACR3 genes in arsenic hyperaccumulator Pteris vittata were studied in yeast and tobacco, showing their roles in regulating arsenic translocation and sequestration. PvACR3;2 facilitated AsIII translocation to the shoots and efflux from the roots in tobacco, while PvACR3;3 sequestered AsIII in tobacco root vacuoles.
Arsenite (AsIII) antiporter ACR3 is crucial for arsenic (As) translocation and sequestration in Ashyperaccumulator Pteris vittata, which has potential for phytoremediation of As-contaminated soils. In this study, two new ACR3 genes PvACR3;2 and PvACR3;3 were cloned from P. vittata and studied in model organism yeast (Saccharomyces cerevisiae) and model plant tobacco (Nicotiana tabacum). Both ACR3s mediated AsIII efflux in yeast, decreasing its As accumulation and enhancing its As tolerance. In addition, PvACR3;2 and PvACR3;3 were expressed in tobacco plant. Localized on the plasma membrane, PvACR3;2 mediated both AsIII translocation to the shoots and AsIII efflux from the roots in tobacco, resulting in 203 - 258% increase in shoot As after exposing to 5 mu M AsIII under hydroponics. In comparison, localized to the vacuolar membrane, PvACR3;3 sequestrated AsIII in tobacco root vacuoles, leading to 18 - 20% higher As in the roots and 15 - 36% lower As in the shoots. Further, based on qRT-PCR, both genes were mainly expressed in P. vittata fronds, indicating PvACR3;2 and PvACR3;3 may play roles in AsIII translocation and sequestration in the fronds. This study provides not only new insights into the functions of new ACR3 genes in P. vittata, but also important gene resources for manipulating As accumulation in plants for phytoremediation and food safety.
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