Journal
JOURNAL OF HAZARDOUS MATERIALS
Volume 404, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.jhazmat.2020.124192
Keywords
DNA-based stable isotope probing; Active triclosan-degrading bacteria; Oligotyping; Metagenomics; Antibiotic resistance genes
Categories
Funding
- Natural Science Foundation of Beijing Municipality [8202006]
- National Natural Science Foundation of China [51578015]
- Beijing Municipal Science and Technology Commission [Z181100005518002]
- National Major Science and Technology Project of China [2017ZX07103-003]
Ask authors/readers for more resources
This study identified the microbial community involved in triclosan degradation during nitrification, with Sphingobium as the predominant species and glutathione transferase as the main enzyme. It was also found that Sphingobium may degrade triclosan without contributing to the spread of antibiotic resistance genes (ARGs). Thauera and Dechloromonas were identified as key hosts of most ARGs in the wastewater treatment system.
The biotransformation of triclosan (TCS) during wastewater treatment occurred frequently, while little researches are known the identity of microorganisms involved in the biodegradation process. In this work, DNA-based stable isotope probing (DNA-SIP) was occupied to investigate the TCS assimilation microbes originated from a full-scale cyclic activated sludge system in Beijing. Results of TCS removal pathway showed that the TCS removal in nitrification process was mainly contributed by the metabolism of heterotrophic bacteria, accounting for about 18.54%. DNA-SIP assay indicated that Sphingobium dominated the degradation of TCS. Oligotyping analysis further indicated that oligotype GCTAAT and ATGTTA of Sphingobium played important roles in degrading TCS. Furthermore, the Kyoto Encyclopedia of Genes and Genomes functional abundance statistics based on PICRUSt2 showed that glutathione transferase was the most prevalent enzyme involved in TCS metabolism, and TCS might be removed through microbial carbon metabolism. Metagenomics made clear that Sphingobium might play irrelevant role on the propagation of antibiotics resistance genes (ARGs), even though, it could degrade TCS. Thauera and Dechloromonas were identified as the key hosts of most ARGs. This study revealed the potential metabolic pathway and microbial ecology of TCS biodegradation in nitrification process of wastewater treatment system.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available