Effects of polystyrene nanoplastics on Ctenopharyngodon idella (grass carp) after individual and combined exposure with zinc oxide nanoparticles

The toxicity of polystyrene nanoparticles (PS NPs) and ZnO nanoparticles (ZnO NPs), in combination is poorly known. Thus, the aim of the current study was to evaluate the effects of PS NPs (760 mu g/L) on Ctenopharyngodon idella exposed to it, both in separate and in combination with ZnO NPs (760 mu g/L), based on behavioral, biochemical and genotoxic biomarkers. Current data have indicated that PS NPs, for a short exposure period (3 days), both in separate and in combination with nanoparticles, have affected animals' response to the mirror test. On the other hand, all treatments have equally induced C. idella inactivity towards alarm substances and DNA damage. There was increased oxidative stress, mainly in groups exposed to PS NPs (in combination, or not, with nanoparticles); although increased, the evaluated antioxidant levels did not appear to be enough to inhibit the effects of treatment-induced production of free radicals. Together, these results are likely co-responsible for the observed changes. The current study did not observe antagonistic, synergistic or additive effect on animals exposed to the combination between PS NPs and ZnO NPs; however, this outcome should not discourage the performance of similar studies focused on assessing the (eco)toxicity of pollutant mixtures comprising nanomaterials.

Automated summary

The study found that short-term exposure to PS NPs affected fish's response to mirror tests, as well as induced inactivity towards alarm substances and DNA damage. Increased oxidative stress was observed, and while antioxidant levels were elevated, they were not sufficient to counteract the effects of free radicals produced by the treatment.