Journal
JOURNAL OF HAZARDOUS MATERIALS
Volume 406, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.jhazmat.2020.124790
Keywords
Lead pollution; Amplification; DNAzyme; Label-free; Fluorescence assay
Categories
Funding
- National Natural Science Foundation of China (China) [22074100]
- Green Manufacturing Project of Ministry of Industry and Information Technology of China (China)
- King Saud University, Riyadh, Saudi Arabia (Saudi Arabia) [RSP-2020/138]
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This study presents a label-free, dually amplified and homogeneous DNAzyme assay for sensitive and one-pot detection of lead pollution, which can precisely detect Pb2+ in tap water, milk and fish, promising for on-site monitoring lead pollution risk and ensuring environmental and food safety.
Lead pollution in water and soil often transfers to food, advocating tools for on-site detection of lead pollution to ensure both environmental and food safety. We proposed a label-free, dually amplified and homogeneous DNAzyme assay for sensitive and one-pot detection of lead pollution. Instead of using chemically modified DNA substrate, a structure-response digestion process was introduced to monitor Pb2+ presence-induced cleavage process of unlabeled substrate, further amplifying the response signals and eliminating the use of labeled DNA probes. The DNAzyme assay allowed to detect Pb2+ as low as 0.12 nM and endued a dynamic range from 0.1 nM to 30 nM. In addition, it can specifically identify Pb2+ among other metal ions. We demonstrated that the DNAzyme assay can precisely detect Pb2+ in tap water, milk and fish. Thus, the DNAzyme assay is promising for on-site monitoring lead pollution risk and ensuring environmental and food safety.
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