Journal
JOURNAL OF FLUORESCENCE
Volume 31, Issue 3, Pages 719-725Publisher
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10895-021-02705-4
Keywords
Xanthene; Viscosity; Fluorescence imaging
Funding
- Natural Science Foundation of China [52009053]
- Key Program of the Educational Department of Sichuan Province, China [18ZA0263]
- Program of Science & Technology Bureau of Mianyang City, China [2020YFZJ005]
- General Program of Science & Technology Department of Sichuan Province, China [2018JY0223]
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ImX, a new xanthene dye, exhibits viscosity-selective fluorescent response and emission enhancement in water. Moreover, it shows low cytotoxicity and can be employed for detection of Monensin-triggered viscosity enhancement.
A new xanthene dye, namely ImX, has been facilely prepared by reaction of 4-(1H-Imidazol-1-yl)benzaldehyde with N, N-diethyl-3-aminophenol in concentrated propionic acid, and then treated by p-chloranil. ImX presents the maximum absorption and emission band centered at 562 nm and 583 nm in water, respectively. Fluorescent spectra investigations demonstrate that ImX shows viscosity-selective fluorescent response and emission enhancement when the solvent viscosity increases from 1.1 cp. (water) to 1248 cp. (98 % glycerol). In addition, this viscosity-selective fluorescence response covers a wide pH range from 2.5 to 10.0. More significantly, ImX demonstrates low cytotoxicity and can be employed as tracer for the detection of Monensin-triggered viscosity enhancement by cell imaging.
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