4.5 Article

Genetically modified attenuated salmonid alphavirus: A potential strategy for immunization of Atlantic salmon

Journal

JOURNAL OF FISH DISEASES
Volume 44, Issue 7, Pages 923-937

Publisher

WILEY
DOI: 10.1111/jfd.13352

Keywords

attenuation; capsid; N‐ glycosylation; pancreas disease; salmonid alphavirus; virulence

Funding

  1. Research Council of Norway [280847/E40]

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The study shows that immunization with targeted mutations in SAV3 strains can lead to PD, but by injecting mutated viral strains, salmon can acquire a certain degree of protection, with reduced clinical symptoms and increased weight gain.
Pancreas disease (PD) is a serious challenge in European salmonid aquaculture caused by salmonid alphavirus (SAV). In this study, we report the effect of immunization of Atlantic salmon with three attenuated infectious SAV3 strains with targeted mutations in a glycosylation site of the envelope E2 protein and/or in a nuclear localization signal in the capsid protein. In a pilot experiment, it was shown that the mutated viral strains replicated in fish, transmitted to naive cohabitants and that the transmission had not altered the sequences. In the main experiment, the fish were immunized with the strains and challenged with SAV3 eight weeks after immunization. Immunization resulted in infection both in injected fish and 2 weeks later in the cohabitant fish, followed by a persistent but declining load of the mutated virus variants in the hearts. The immunized fish developed clinical signs and pathology consistent with PD prior to challenge. However, fish injected with the virus mutated in both E2 and capsid showed little clinical signs and had higher average weight gain than the groups immunized with the single mutated variants. The SAV strain used for challenge was not detected in the immunized fish indicating that these fish were protected against superinfection with SAV during the 12 weeks of the experiment.

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