Journal
JOURNAL OF CLINICAL MICROBIOLOGY
Volume 59, Issue 4, Pages -Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.02438-20
Keywords
ELISA; SARS-CoV-2; neutralizing antibodies; serology; vaccines
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The cPass surrogate virus neutralization test (sVNT) is a highly accurate tool for monitoring SARS-CoV-2 immunity, providing valuable assistance in surveillance of infected/recovered and/or vaccinated individuals, as well as in drug and convalescent-phase donor screening. The data also suggest potential applications for calibrating live-cell neutralization tests and longitudinal testing of recovered and/or vaccinated patients.
Many severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) serology tests have proven to be less accurate than expected and do not assess antibody function as neutralizing, correlating with protection from reinfection. A new assay technology measuring the interaction of the purified SARS-CoV-2 spike protein receptor binding domain (RBD) with the extracellular domain of the human angiotensin-converting enzyme 2 (hACE2) receptor detects these important antibodies. The cPass surrogate virus neutralization test (sVNT), compared directly with eight SARS-CoV-2 IgG serology and two live-cell neutralization tests, gives similar or improved accuracy for qualitative delineation between positive and negative individuals in a fast, scalable, and high-throughput assay. The combined data support the cPass sVNT as a tool for highly accurate SARS-CoV-2 immunity surveillance of infected/recovered and/or vaccinated individuals as well as drug and convalescent-phase donor screening. The data also preview a novel application for the cPass sVNT in calibrating the stringency of live-cell neutralization tests and its use in longitudinal testing of recovered and/or vaccinated patients.
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