4.6 Article

Determination of activity of denitrifying enzyme in soil samples by a headspace gas chromatographic technique

Journal

JOURNAL OF CHROMATOGRAPHY A
Volume 1638, Issue -, Pages -

Publisher

ELSEVIER
DOI: 10.1016/j.chroma.2021.461882

Keywords

Headspace Gas Chromatography; Denitrifying enzyme; Nitrite nitrogen; Nitrogen dioxide

Funding

  1. National Key and Development Program of China [2018YFC1902102]
  2. Key Project of the Science and Technology Ministry of Nanning, China [20183045-2]

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This paper presents a headspace gas chromatography method for determining denitrifying enzyme activity in soil samples, which demonstrates good measurement precision and reasonable accuracy, making it suitable for batch sample analysis.
This paper reported a headspace gas chromatography (HS-GC) for the determination of denitrifying enzyme activity in soil samples. It was based on measuring the NO2 signal in a set of closed/air-free vials containing soil samples that incubated at the designated conditions. The results showed that the method has a good measurement precision (RSD < 5.0%) and reasonable accuracy for determining the denitrifying enzyme activity in soil samples. It is simple and efficient, and very suitable to be used in batch sample analysis. (C) 2021 Elsevier B.V. All rights reserved.

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