Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 69, Issue 7, Pages 2118-2128Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.0c06677
Keywords
egg white; ultrafiltration; antimicrobial activity; peptide; Salmonella Enteritidis
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Recent research has shown that egg white at 45 degrees C has powerful bactericidal activity against S. enterica serovar Enteritidis, partly due to two specific peptides. However, these peptides only contribute to part of the bactericidal activity, suggesting the presence of other yet to be determined antimicrobial factors.
A recent work revealed that egg white (EW) at 45 degrees C exhibits powerful bactericidal activity against S. enterica serovar Enteritidis, which is surprisingly little affected by removal of the >10 kDa EW proteins. Here, we sought to identify the major EW factors responsible for this bactericidal activity by fractionating EW using ultrafiltration and nanofiltration and by characterizing the physicochemical and antimicrobial properties of the resulting fractions. In particular, 22 peptides were identified by nano-LC/MS-MS and the bactericidal activities of representative peptides (with predicted antimicrobial activity) were further assessed. Two peptides (FVPPVQR and GDPSAWSWGAEAHS) were found to be bactericidal against S. enterica serovar Enteritidis at 45 degrees C when provided in an EW environment. Nevertheless, these peptides contribute only part of this bactericidal activity, suggesting other, yet to be determined, antimicrobial factors.
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