Dense array of floating water droplets aligned to an assembly of tubular wells

A dense array of surfactant-stabilized water droplets (20 mu m in diameter) floating on the surface of fluorinated oil was prepared. More than 30000 droplets were planarly arrayed in an 8 mm squared area using the droplet guide, an assembly of tubular wells located on the top wall of the fluidic channel. Parallel measurement of the melting temperatures of digitally partitioned KRAS DNAs hybridized with molecular beacons was demonstrated using the array of floating droplets.

Automated summary

This study successfully arranged a dense array of surfactant-stabilized water droplets on the surface of fluorinated oil, and demonstrated parallel measurement of the melting temperatures of digitally partitioned KRAS DNAs hybridized with molecular beacons using the droplet guide.