Lentzea alba sp. nov., a novel actinobacterium isolated from soil

A novel actinobacterium, designated strain NEAU-D13(T), was isolated from soil collected from Aohan Banner, Chifeng, Inner Mongolia Autonomous Region, China and characterized using a polyphasic approach. On the basis of 16S rRNA gene sequence analysis, strain NEAU-D13(T) belonged to the genus Lentzea and shared the highest sequence similarity with Lentzea kentuckyensis JCM 14913(T) (99.17 %). Morphological and chemotaxonomic characteristics of the strain also supported its assignment to the genus Lentzea. Cell walls contained meso-diaminopimelic acid as the diagnostic diamino acid and the whole-cell sugars were ribose and mannose. The phospholipid profile contained diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine and phosphatidylinositol. The menaquinone was only MK-9(H-4). The major fatty acids were iso-C-16:0, C-16:0, anteiso-C-17:0, iso-C-15:0 and anteiso-C-15:0. DNA G+C content was 68.71 mol%. Phylogenetic analysis using the 16S rRNA gene sequences showed that the strain formed a stable clade with L. kentuckyensis JCM 14913(T) in the genus Lentzea. Meanwhile, a combination of digital DNA-DNA hybridization results and some phenotypic characteristics demonstrated that strain NEAU-D13(T) could be distinguished from its closely related strain. Therefore, it is concluded that strain NEAU-D13(T) represents a novel species of the genus Lentzea, for which the name Lentzea alba sp. nov. is proposed, with NEAU-D13(T) (=CCTCC AA 2019089(T)=JCM 33970(T)) as the type strain.

Automated summary

Strain NEAU-D13(T) is a novel actinobacterium isolated from soil in Inner Mongolia, belonging to the genus Lentzea. Its unique morphological and chemotaxonomic characteristics distinguish it from closely related strains.