Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 180, Issue -, Pages 88-96Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2021.02.194
Keywords
Polyelectrolytic complexes; Non-toxic anticancer active principle; Chondroitin sulfate; Hydrogel application; Curcumin; Cancer cells apoptosis; Imidazolium salt
Funding
- CNPq - Conselho Nacional de Desenvolvimento Cientifico e Tecnologico [307429/2018-0]
- CAPES - Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior
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Chitosan/chondroitin sulfate (CHT/CS) cure min-charged hydrogels prepared through polyelectrolytic complexation (PEC) show significant anticancer effects, with PEC-T-CUR method being more effective than PEC-CUR method for treating HeLa cancer cells. PECs exhibit favorable characteristics, such as particle size, hydrophobicity, and pH swelling, with CUR entrapment levels reaching around 100%.
Chitosan/chondroitin sulfate (CHT/CS) cure min-charged hydrogels were prepared through polyelectrolytic complexation (PEC) following two methodologies (PEC-CUR and PEC-T-CUR) and were applied on apoptosis of HeLa, HT29 and PC3 cancer cells. PEC-T-CUR (ionic liquid (IL) mixed using ultraturrax homogenizer) results show to be far better than for PEC-CUR (IL mixed using magnetic stirring), with IC50 being improved 5.13 times to HeLa cancer cells (from 1675.2 to 326.7 mu g mL(-1)). PECs produced by this methodology presented favorable characteristics, such as particle size, hydrophobicity, pH swelling. Beyond this, the IL was quantitatively recovered in both cases. CUR entrapment levels were hugely loaded into PEC at around 100%. Swelling, dissolution/degradation, and pH(pzc )assays showed that PECs may positively act in several environments, releasing the CUR, the CHT and CS as well. Characterization through FTIR, SEM, TEM, TGA, DSC, and WAXS confirmed CUR presence in both types of PECs, and cytotoxic studies showed the significant anticancer effects of CUR-containing PECs. (C) 2021 Elsevier B.V. All rights reserved.
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