Preparation and characterization of ferulic acid-modified water soluble chitosan and poly (γ-glutamic acid) polyelectrolyte films through layer-by-layer assembly towards protein adsorption

In this study, ferulic acid-modified water soluble chitosan and poly (gamma-glutamic acid) polyelectrolyte multilayers films were constructed through the layer-by-layer (LBL) self-assembly technique. Chitosan (CS) or ferulic acid modified chitosan (MCS) and Poly (gamma-glutamic acid) (PGA) was alternately deposited on the surface of glass substrate for the enhancement of surface modification. The obtained films were characterized by Fourier transform spectroscopy (FTIR), X-ray diffractometry (XRD), scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS), UV-vis spectroscopy and water contact angle to study its physico-chemical properties including protein absorption. The (PGA/MCS) films showed intense deposition of multilayers built upon the surface roughness and an increase in the exponential growth of multilayer films by UV-vis spectroscopy. Water contact angle indicated that the (PGA/MCS) films performed well with good wettability due to the increase in the number of layers. The LBL multilayer coatings of (PGA/MCS) films surface possessed a reduced amount of protein adsorption. These results indicated that it can resist the protein adsorption and can enhance the biocompatibility towards the biomedical application through the protein interaction. The (PGA/MCS) films has the potential to utilization as a good biomaterial for biomedical purposes to intensify the bio-active surface. (C) 2021 Elsevier B.V. All rights reserved.

Automated summary

In this study, ferulic acid-modified water soluble chitosan and poly (gamma-glutamic acid) polyelectrolyte multilayers films were constructed using layer-by-layer self-assembly technique. The films showed good wettability and protein adsorption resistance, indicating their potential as biomaterials for biomedical applications.