Journal
INNATE IMMUNITY
Volume 27, Issue 3, Pages 230-239Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1177/1753425921994190
Keywords
Portulaca oleracea L; zymosan; inflammation; Nrf2; NLRP3
Categories
Funding
- Nature Science Fund of Hunan Province [2018JJ2346, 2018JJ2348]
Ask authors/readers for more resources
The study aimed to investigate the effects of ethanol extracts from Portulaca oleracea L. (ePO) on joint inflammation and their underlying mechanisms. Results showed that ePO pre-treatment inhibited articular mechanical hyperalgesia and edema, improved pathological alternations by reducing inflammatory cells, regulated Nrf2-related proteins to inhibit oxidative stress, and attenuated joint inflammation by modulating the NLRP3 inflammasome and NF-kappa B activation.
The aim of this study was to explore the effects of ethanol extracts from Portulaca oleracea L. (ePO) on joint inflammation and to explain the underlying mechanisms. A joint inflammation mouse model was constructed by injecting zymosan, and the Von Frey method was employed and the joint thickness measured. The numbers of leukocytes, neutrophils, and monocytes were counted in the joint cavity and the infiltration of inflammatory cells was assessed by joint histopathological analysis. The mRNA levels of inflammatory cytokines were determined by quantitative RT-PCR and their secretion levels were determined by specific ELISAs. Pre-treatment with ePO inhibited articular mechanical hyperalgesia and edema and ameliorated the recruitment of mononuclear neutrophils and leukocytes. In addition, pre-treatment with ePO improved pathological alternations in the joint tissues by reducing the number of inflammatory cells. Pre-treatment with ePO regulated the nuclear factor erythroid 2-related factor 2 (Nrf2)-related proteins and thereby inhibited oxidative stress. In addition, ePO inhibited NLR family pyrin domain containing 3 (NLRP3) inflammasome-related genes (NLRP3, ASC, pro-caspase-1 and pro-IL-1ss), modulated inflammatory cytokines and the activation of NF-kappa B. ePO attenuated zymosan-induced joint inflammation by regulating oxidative stress, NLRP3 inflammasome, and NF-kappa B.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available