Highly Sensitive Electrochemical Impedance- Based Biosensor for Label-Free and Wide Range Detection of Fibrinogen Using Hydrothermally Grown AlFeO3 Nanospheres Modified Electrode

We report an ultra-selective biosensor for detection of fibrinogen (fib) using AlFeO3 nanospheres modified screen-printed carbon electrode (AFO/SPCE) via electrochemical impedance spectroscopy (EIS). The orthorhombic phase and nanospheres-like morphology of hydrothermally grown AlFeO3 are confirmed via X-Ray diffraction (XRD) and scanning electron microscopy (SEM) imaging, respectively. The sensor exhibits excellent selectivity, sensitivity and low limit of detection (LOD) of 14 ng mL(-1) with a wide dynamic range from 3 mu g mL(-1) to 340 x 103 mu g mL(-1) which is more suitable for detecting both the deficiency and excess level of fib in the human blood. The decrease in charge transfer resistance (Rct) with an increase in fib concentrations can be attributed to the strong interaction and electrooxidation of fib molecules forming protein carbonyl groups at the surface of the electrodewith enhanced electrical conductivity. This is a report on the low-cost, simple electrochemical impedance sensor using perovskite AlFeO3 for reliable and rapid sensing of fibwhich shows great potential for developing biosensors for healthcare.

Automated summary

The study reported an ultra-selective biosensor for fibrinogen detection using AlFeO3 nanospheres modified screen-printed carbon electrode, which exhibited excellent selectivity and sensitivity. The sensor has a low limit of detection and is suitable for detecting both deficiency and excess levels of fibrinogen in human blood.