Journal
GENOME BIOLOGY AND EVOLUTION
Volume 13, Issue 3, Pages -Publisher
OXFORD UNIV PRESS
DOI: 10.1093/gbe/evab024
Keywords
two-component system; MORC; GHKL ATPases; DNA modification methylases; DNA restriction-modification enzymes; histidine kinase
Categories
Funding
- National Research Foundation (NRF), South Africa through Competitive Funding for Rated Researchers (CFRR) [98993]
- Research Technology Fund (RTF) [98654]
- University of Pretoria Postdoctoral Fellowship
Ask authors/readers for more resources
Two newly predicted protein domains, Response_reg_2 and HEF_HK, show high structural similarity with typical domains of response regulators (RRs) and histidine kinases (HKs), respectively, suggesting a potential new two-component system (TCS) in bacteria. The genes encoding these domains exhibit a high linkage, indicating a possible functional association and evolutionary relationship with known HKs and RRs. Additionally, the genomic regions where these predicted systems are located show overrepresentation of restriction-modification (R-M) systems and GHKL family domain HATPase_c_3, further supporting their potential role in bacterial signaling pathways.
Two-component systems (TCS) are important types of machinery allowing for efficient signal recognition and transmission in bacterial cells. The majority of TCSs utilized by bacteria is composed of a sensor histidine kinase (HK) and a cognate response regulator (RR). In the present study, we report two newly predicted protein domains-both to be included in the next release of the Pfam database: Response_reg_2 (PF19192) and HEF_HK (PF19191)-in bacteria which exhibit high structural similarity, respectively, with typical domains of RRs and HKs. Additionally, the genes encoding for the novel predicted domains exhibit a 91.6% linkage observed across 644 genomic regions recovered from 628 different bacterial strains. The remarkable adjacent colocalization between genes carrying Response_reg_2 and HEF_HK in addition to their conserved structural features, which are highly similar to those from well-known HKs and RRs, raises the possibility of Response_reg_2 and HEF_HK constituting a new TCS in bacteria. The genomic regions in which these predicted two-component systems-like are located additionally exhibit an overrepresented presence of restriction-modification (R-M) systems especially the type II R-M. Among these, there is a conspicuous presence of C-5 cytosine-specific DNA methylases which may indicate a functional association with the newly discovered domains. The solid presence of R-M systems and the presence of the GHKL family domain HATPase_c_3 across most of the HEF_HK-containing genes are also indicative that these genes are evolutionarily related to the paraMORC family of ATPases.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available