Structure, gene order, and nucleotide composition of mitochondrial genomes in parasitic lice from Amblycera

Parasitic lice have unique mitochondrial (mt) genomes characterized by rearranged gene orders, variable genome structures, and less AT content compared to most other insects. However, relatively little is known about the mt genomes of Amblycera, the suborder sister to all other parasitic lice. Comparing among nine different genera (including representative of all seven families), we show that Amblycera have variable and highly rearranged mt genomes. Some genera have fragmented genomes that vary considerably in length, whereas others have a single mt chromosome. Notably, these genomes are more AT-biased than most other lice. We also recover genus-level phylogenetic relationships among Amblycera that are consistent with those reported from large nuclear datasets, indicating that mt sequences are reliable for reconstructing evolutionary relationships in Amblycera. However, gene order data cannot reliably recover these same relationships. Overall, our results suggest that the mt genomes of lice, already know to be distinctive, are even more variable than previously thought.

Automated summary

Parasitic lice have unique mitochondrial genomes characterized by rearranged gene orders, variable structures, and less AT content compared to other insects. The mitochondrial genomes of Amblycera are highly rearranged and AT-biased, with genus-level phylogenetic relationships being consistent with large nuclear datasets but gene order data being unreliable in recovering these relationships. Overall, the mt genomes of lice are more variable than previously thought.