Journal
FOOD SCIENCE AND BIOTECHNOLOGY
Volume 30, Issue 2, Pages 245-256Publisher
KOREAN SOCIETY FOOD SCIENCE & TECHNOLOGY-KOSFOST
DOI: 10.1007/s10068-020-00870-z
Keywords
Lactobacillus reuteri TF-7; Probiotics; Spray-drying microencapsulation; Nano-crystalline starch; Whey protein isolate
Categories
Funding
- Research and Researchers for Industries Program
- Thailand Science Research and Innovation [PHD61I0020]
- Faculty of Medicine, Srinakharinwirot University [219/2560]
- HRH Princess Maha Chakri Sirindhorn Medical Center, Srinakharinwirot University [219/2560]
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The study microencapsulated Lactobacillus reuteri TF-7 producing bile salt hydrolase in whey protein isolate (WPI) or whey protein isolate combined with nano-crystalline starch (WPI-NCS) using spray-drying technique to enhance probiotic survivability and stability. The WPI-NCS microcapsules showed significantly higher survival rates under adverse conditions and were able to retain both survival and biological activity during long-term storage. Microcapsules fabricated from WPI-NCS showed great potential for developing cholesterol-lowering probiotic supplements.
Decrease of survivability and stability is a major problem affecting probiotic functional food. Thus, in this study, Lactobacillus reuteri TF-7 producing bile salt hydrolase was microencapsulated in whey protein isolate (WPI) or whey protein isolate combined with nano-crystalline starch (WPI-NCS) using the spray-drying technique to enhance the survivability and stability of probiotics under various adverse conditions. Spherical microcapsules were generated with this microencapsulation technique. In addition, the survival of L. reuteri TF-7 loaded in WPI-NCS microcapsules was significantly higher than WPI microcapsules and free cells after exposure to heat, pH, and simulated gastrointestinal conditions. During long-term storage at 4, 25, and 35 degrees C, WPI-NCS microcapsules could retain both survival and biological activity. These findings suggest that microcapsules fabricated from WPI-NCS provide the most robust efficiency for enhancing the survivability and stability of probiotics, in which their great potentials appropriate to develop as the cholesterol-lowering probiotic supplements.
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