Journal
FOOD RESEARCH INTERNATIONAL
Volume 140, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.foodres.2020.109865
Keywords
Multiclass multiresidue method; QuEChERS approach; LC-QqQ-MS/MS; Veterinary drugs; Antimicrobial drugs; Antibiotic residues in fish; Tilapia; Food surveillance
Categories
Funding
- Sao Paulo Research Foundation-Agilent Technologies [2013/50452-5]
- Coordination for the Improvement of Higher Level Education Personnel (CAPES/PROEX) [001]
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This study validated an LC-MS/MS method to detect residues of 25 antimicrobial drugs in tilapia fillet samples from Brazil, identifying residues of oxytetracycline, florfenicol, and enrofloxacin. The findings highlight the need for attention to the use of veterinary products in fish farming in Brazil.
This study was carried out to assess the occurrence of antimicrobial residues in samples of tilapia (Oreochromis niloticus) fillets collected at the State of Sao Paulo retail market and produced from fish farmed in Brazil. For this purpose, a liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) method was validated and used to quantify residues of 25 antibacterial drugs (2 beta-lactams, 8 quinolones, 2 macrolides, 5 sulfonamides, 4 tetracyclines, 3 amphenicols and 1 sulfonamide potentiator). For the sample preparation step the QuEChERS approach was performed. Chromatographic separation was conducted using a Zorbax SB C18 column. Method validation was performed based on European and Brazilian guidelines. The validation parameters (linearity, intra- and inter-day precision, accuracy, decision limit, detection capability and robustness) attended the adopted validation guidelines. Limits of detection and quantitation were also determined. Antimicrobial drug residues were quantitated in the incurred samples by using matrix-matched analytical curves. Oxytetracycline, florfenicol and, for the first time, enrofloxacin residues are reported in tilapia fillet samples from Brazil, though, in accordance with the European and Brazilian regulatory framework. Thus, our results draw attention to the use of veterinary products in fish farming in Brazil. Monitoring of veterinary drug residues is essential to ensure the safety of fish products available to the consumer, as well as to keep fish as a food commodity.
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