Journal
FOOD HYDROCOLLOIDS
Volume 112, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodhyd.2020.106293
Keywords
Soy isolated protein; Tannic acid; Covalent bond; Hydrogels
Categories
Funding
- Heilongjiang Provincial Natural Science Foundation of China [ZD 2019C002]
- National Key Research and Development Program of China [2017YFC1601901]
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Covalent cross-linking of soy protein isolate (SPI-12%) with tannic acid (TA-29,58,88,117,146 mu mol/g SPI) in an alkaline environment was found to improve the structure, antioxidant activity, and gelation characteristics of SPI-TA conjugates. The addition of TA initiated irreversible SPI modifications, resulting in optimal hardness and elasticity at pH 11, and increased storage and loss moduli with higher TA concentrations. Analysis shows that higher TA concentration leads to a more compact network structure, indicating pH-dependent interactions between SPI and TA modify gel formation to enhance rheological and textural properties of the gels.
The effects of covalent cross-linking of soy protein isolate (SPI-12%) with tannic acid (TA-29,58,88,117,146 mu mol/g SPI) in an alkaline environment on the structure, antioxidant activity and gelation characteristics of SPI-TA conjugates were found to improve all properties. After alkali treatment, the tryptophan content in the conjugate decreased and the covalent binding rate increased. The addition of TA initiated irreversible SPI modifications, as shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), Fourier transform infrared (FTIR) spectroscopy, fluorescence spectroscopy and thermogravimetry. The texture analysis of the gel showed that hardness and elasticity were optimal at pH 11, and the rheological properties of the storage modulus (G') and loss modulus (G '') increased with higher TA concentrations. Analysis of the microstructure indicates that an increase in TA concentration results in a more compact network structure. Our results indicate that pH dependent interactions between SPI and TA modify the formation of the gel in a way that improves the rheology and textural properties of the gels.
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