Application of LF-NMR to the characterization of camellia oil-loaded pickering emulsion fabricated by soy protein isolate

In this study, the camellia oil-loaded Pickering emulsion stabilized by soybean protein isolate (SPI) was selected, and low-field nuclear magnetic resonance were adopted to characterize the emulsion transformation process besides conventional characterization index. Results showed that the T-2 distribution of SPI dispersion only showed one hydrogen pool, and T-cur, T-2W and T-2 all decreased with the increase of SPI concentration, indicating faster decay and lower proton mobility, which was companied by increasing particle diameter. The addition of NaCl and heating (95 degrees C, 15 min) can speed up the relaxation decay, increase the surface hydrophobicity and reduce the nanoparticle diameter. As for the emulsion, three hydrogen populations were observed on T-2 distribution. Based on the correlation results, the hydrogen pools were successfully attributed to oil and water phase, respectively. Meanwhile, with the increase of oil volume fraction (0.1-0.6), the T-cur and T-2W tended to decrease, the absorbed protein percentage (AP%) and the shear viscosity tended to increase, while the droplet diameter only increased significantly when phi was below 0.3. Lastly, the T-2 distribution of the oleogel showed two hydrogen populations, and the population (T-22) could be interpreted as the mobility of protons in oil molecules, which accounted for more than 95% of the total signal. Although the elastic properties of oleogel became weaker with the increase of phi, the T-cur and T-2W were relatively stable. However, it was significantly distinctive from that of SPI dispersion and emulsion. In summary, LF-NMR could be used for the rapid characterization of emulsion.

Automated summary

In this study, the camellia oil-loaded Pickering emulsion stabilized by soybean protein isolate (SPI) was characterized using low-field nuclear magnetic resonance. The results showed that increasing SPI concentration led to faster decay and lower proton mobility, as well as increasing particle diameter. The addition of NaCl and heating could accelerate relaxation decay and alter nanoparticle diameter.