Journal
FOOD CHEMISTRY
Volume 355, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2021.129443
Keywords
Carbon dots; Inner filter effect; Immunosensor; Milk; Aflatoxin M1
Funding
- National Natural Science Foundation of China [31972763, 31602103]
- Natural Science Foundation of Guangdong Province, China [2019A1515011834]
- Science and Technology Planning Project of Guangdong Province, China [2017B020207009]
- Shenzhen Basic Research Projects [JCYJ20190808160003705, JCYJ20170817095240632]
- medical young scientists program in Shenzhen University
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A facile hydrothermal method was used to synthesize highly photoluminescent N-doped carbon dots with quantum yields reaching 97.1%. An label-free immunosensor based on the inner filter effect of carbon dots was developed for ultrasensitive detection of aflatoxin M1 residues in milk, with a detection limit of 0.0186 ng/mL. The immunosensor showed good linear relationship, high recoveries, and low relative standard deviations, making it a reliable on-site screening method for aflatoxin M1 residue analysis.
Here, a facile hydrothermal method was used to synthesize highly photoluminescent N-doped carbon dots, and the quantum yields reached 97.1%. Then, a label-free immunosensor based on the inner filter effect of carbon dots was developed for ultrasensitive detection of aflatoxin M1 residues in milk. The detection limit was 0.0186 ng/mL (equivalents to 18.10 ng/kg), which satisfied the most stringent maximum tolerable limit value of 25 ng/ kg. Besides, the immunosensor showed a good linear relationship from 0.003 ng/mL to 0.81 ng/mL, and the average recoveries ranged from 79.6% to 112.5% for spiked milk samples, with relative standard deviations ranging from 6.7% to 13.3%. Compared with other immunoassays, the inner filter effect-based immunosensor incorporating fluorescent detection into conventional enzymatic cascade amplification systems and could be a reliable on-site screening method for aflatoxin M1 residue analysis.
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