Extraction of antioxidant peptides from rice dreg protein hydrolysate via an angling method

Selective enrichment of the highly active antioxidant peptides is required as the lack of an efficient method leads to long screening processes, hampering the research of antioxidant peptides. A simple synthetic metal-organic framework MIL-53 (Cr) was initially applied to extract specific antioxidant peptides from rice dreg protein hydrolysate. The highest active fraction was further purified by reversed-phase high-performance liquid chromatography. The antioxidant peptides with the highest antioxidant activities were identified as Gly-Asp-Met-Asn-Pro and Leu-Leu-Leu-Arg-Trp by LC-MS. These two peptides were synthesized and also exhibited good scavenging activity on the DPPH free radical, superoxide anion free radical and hydroxyl radical, and good chelating ability on Fe2+. The results confirmed that the angling method was effective for antioxidant peptide enrichment from protein hydrolysates.

Automated summary

Selective enrichment of antioxidant peptides with high activity is essential due to the lack of efficient methods leading to long screening processes. The use of a synthetic metal-organic framework and reversed-phase high-performance liquid chromatography successfully extracted two antioxidant peptides with the highest antioxidant activities from rice dreg protein hydrolysate.