A simple and sensitive sensor for lactose based on cascade reactions in Au nanoclusters and enzymes co-encapsulated metal-organic frameworks

In this work, one kind of zeolite imidazole frameworks containing bovine serum albumin stabilized Au nanoclusters (AuNCs), beta-galactosidase (beta-Gal) and glucose oxidase (GOx) (AuNCs/beta-Gal/GOx@ZIF-8) were obtained to detect lactose. Compared with other fluorescent nano-materials, AuNCs show distinct advantages as a guest species in ZIF-8, specifically their extremely small size ( < 1 nm), simple synthesis, excellent biocompatibility and high stability. Furthermore, the bovine serum albumin on their surfaces can promote the formation of ZIF-8 coating; thus, AuNCs were co-encapsulated in ZIF-8 with the enzymes together. X-ray diffraction (XRD) analysis indicates the composite possesses the similar crystalline structure with pure ZIF-8. Fluorescence microscope images, Fourier transform infrared spectra and energy dispersive X-ray spectroscopy indicate the presence of AuNCs in the composite. Owing to the high local concentrations of the fluorescent probe and the quenching agent in AuNCs/beta-Gal/GOx@ZIF-8, the quenching rate was enhanced 3.4-fold that of free AuNCs and enzymes in solution.

Automated summary

This work involved the utilization of a zeolite imidazole framework containing AuNCs, β-Gal, and GOx to detect lactose, leveraging the advantages of extremely small size, excellent biocompatibility, and high stability. Analysis indicated that the composite possessed a crystalline structure similar to pure ZIF-8. The high local concentrations of the fluorescent probe and quenching agent in AuNCs/β-Gal/GOx@ZIF-8 led to a 3.4-fold increase in quenching rate compared to free AuNCs and enzymes in solution.