Journal
FOOD CHEMISTRY
Volume 339, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2020.128091
Keywords
Melatonin; Trans-resveratrol; Wine; Microextraction; Green chemistry; Method validation; Response surface methodology
Funding
- FCT/MCTES through national funds [PTDC/SAU-NUT/30322/2017]
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The discovery of melatonin in wines led to a new interest in health benefits and wine consumption, often attributed to trans-resveratrol. A new method for analyzing melatonin and trans-resveratrol in wines using dispersive liquid-liquid microextraction was developed, showing excellent linearity, repeatability, and accuracy. The method was successfully applied to various wine varieties, highlighting its overall advantages compared to existing methods.
The discovery of melatonin (Mel) in wines triggered a new interest in the paradigm of health benefits and wine consumption, usually ascribed to trans-resveratrol (trans-RSV). In this context, a dispersive liquid-liquid microextraction for the analysis of Mel and trans-RSV in wines by LC-FLD was developed. A 2(6-1) factorial design was used to identify the significant variables (p < 0.05) and Central Composite Design was used to achieve the optimal conditions: 300 mu L of chloroform (extracting solvent), 1500 tL of acetonitrile (disperser solvent) and 1500 mg of NaCl (ionic strength). Excellent linearity (R-2 > 0.9999), repeatability (< 3.55%), and accuracy (< 7.18%) were obtained using a blank matrix and recoveries (> 91.9%) using wines. The method was successfully applied to the analyses of Mel (0.63-7.44 ng mL(-1)) and trans-RSV (169-2616 ng mL(-1)) in different wine varieties. Comparison with literature point the overall advantages of the new method.
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