Bioactive flavonoids from Rubus corchorifolius inhibit α-glucosidase and α-amylase to improve postprandial hyperglycemia

This research established an optimized method for the extraction and enrichment of flavonoids from R. corchorifolius fruit. Under the optimized extraction conditions, 12 flavonoids (1-12) were isolated, of which six (2-4, 9-10, 12) were obtained from R. corchorifolius for the first time. Compound 4 showed significant alpha-glucosidase (4.96 mu M) and alpha-amylase (8.04 mu M) inhibitory effects. Molecular modeling revealed that compound 4 exhibits strong binding with the active sites of alpha-glucosidase and alpha-amylase. Lineweaver-Burk plot analysis and surface plasmon resonance revealed the possible dynamic binding mechanism of the flavonoids with alpha-glucosidase and alpha-amylase. The enriched flavonoids and compound 4 showed significant hypoglycemic effects in mice administered a high dose of glucose. In this study, a variety of flavonoids with hypoglycemic activity were found for the first time, revealing the rich chemical composition of R. corchorifolius fruit and highlighting the potential value of R. corchorifolius fruit flavonoids as dietary supplements.

Automated summary

This study established an optimized method for extracting and enriching flavonoids from R. corchorifolius fruit, isolating and identifying 12 flavonoids, six of which were obtained for the first time from R. corchorifolius. Compound 4 in particular showed significant inhibitory effects on alpha-glucosidase and alpha-amylase, with molecular modeling revealing its strong binding with the active sites of the enzymes. The enriched flavonoids and compound 4 demonstrated significant hypoglycemic effects in mice, highlighting the potential value of R. corchorifolius fruit flavonoids as dietary supplements.