Validation of Quick Polar Pesticides (QuPPe) Method for Determination of Eight Polar Pesticides in Cherries by LC-MS/MS

A novel and sensitive chromatography method with modified Quick Polar Pesticide (QuPPe) sample preparation was validated for determination of eight high polar pesticides in cherries by liquid chromatography-triple quadrupole mass spectrometry (LC-MS/MS). The validation data executed the conditions of the European SANTE/12682/2019 Guidance Document. Good linearity (R-2 > 0.99) was observed, and the residuals were lower than +/- 20%. Average recoveries ranged from 70.2 to 105.1%, and LOQ values ranged from 1.77 to 12.13 mu g kg(-1). The repeatability and reproducibility are in range of 1.57-15.56% and 3.51-16.17% separately. All measurement uncertainty values were lower than 50% for all polar pesticides. This verified analytical method was used to detect pesticide residue levels of cherries, and none of the eight high polar pesticides were found in 225 real samples. This is the first validation report of QuPPe sample preparation and LC-MS/MS method of high polar pesticide residues in cherries from Turkey.

Automated summary

A novel and sensitive chromatography method using modified QuPPe sample preparation was validated for determination of eight high polar pesticides in cherries. The method showed good linearity, low residuals, and average recoveries ranging from 70.2 to 105.1%. No high polar pesticides were found in 225 real samples of cherries analyzed using this method.