Journal
FISH PHYSIOLOGY AND BIOCHEMISTRY
Volume 47, Issue 2, Pages 565-581Publisher
SPRINGER
DOI: 10.1007/s10695-021-00932-x
Keywords
Transcriptome; Sex-related genes; Differential expression; Sex reversal; Pseudobagrus ussuriensis
Categories
Funding
- Fishery Science and Technology Projects in Jiangsu Province [2018-23]
- Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection [HSXT2-216]
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The Ussuri catfish has an XX/XY sex determination system, with the genes for sex determination remaining unknown. Transcriptome analysis revealed potential key genes like dmrt1 and gdf9, while also identifying other sex-related genes involved in spermatogenesis, oogenesis, and gonadal development/differentiation. These findings provide valuable insights for future research and for potentially enhancing aquacultural productivity by producing monosex Ussuri catfish.
The Ussuri catfish (Pseudobagrus ussuriensis) has an XX/XY sex determination system but its sex determination gene(s) remain unknown. To better understand the molecular sex determination mechanism, transcriptome analysis was conducted to obtain sex-related gene expression profiles. Transcriptome analyses were made of male and female developing/differentiating gonads by high-throughput RNA sequencing, including gonads from fish given an estradiol-induced sex reversal treatment. A total of 81,569 unigenes were assembled and 39,904 were significantly matched to known unique proteins by comparison with public databases. Twenty specifically expressed and 142 differentially expressed sex-related genes were extracted from annotated data by comparing the treatment groups. These genes are involved in spermatogenesis (e.g., Dnali1, nectin3, klhl10, mybl1, Katnal1, Eno4, Mns1, Spag6, Tsga10, Septin7), oogenesis (e.g., Lagr5, Fmn2, Npm2, zar1, Fbxo5, Fbxo43, Prdx4, Nrip1, Lfng, Atrip), gonadal development/differentiation (e.g., Cxcr4b, Hmgb2, Cftr, Ch25h, brip1, Prdm9, Tdrd1, Star, dmrt1, Tut4, Hsd17b12a, gdf9, dnd, arf1, Spata22), and estradiol response (e.g., Mmp14, Lhcgr, vtg1, vtg2, esr2b, Piwil1, Aifm1, Hsf1, gdf9). Dmrt1 and gdf9 may play an essential role in sex determination in P. ussuriensis. The expression patterns of six random genes were validated by quantitative real-time PCR, which confirmed the reliability and accuracy of the RNA-seq results. These data provide a valuable resource for future studies of gene expression and for understanding the molecular mechanism of sex determination/differentiation and gonadal development/differentiation (including hormone-induced sexual reversal) in Ussuri catfish. This has the potential to assist in producing monosex Ussuri catfish to increase aquacultural productivity.
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