Journal
EXPERIMENTAL PARASITOLOGY
Volume 224, Issue -, Pages -Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.exppara.2021.108098
Keywords
Schistosoma japonicum; DNA dipstick; LAMP; qPCR; Point-of-care testing
Categories
Funding
- NationalHealth and Medical Research Council of Australia [APP1102926, APP1098244, APP1132975, APP1160046, APP1194462]
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Researchers have optimized DNA dipstick technology to quickly purify Schistosoma japonicum DNA, successfully detecting it in various life stages and enabling on-site disease detection.
Molecular diagnostics are powerful tools for disease detection but are typically confined to the laboratory environment due to the cumbersome methods required to extract nucleic acids from biological samples. Accurate diagnosis is essential for early detection of parasitic worm infections and for monitoring control programs, particularly during new transmission outbreaks to limit infection spread. We optimized the recently developed DNA dipstick technology to purify Schistosoma japonicum DNA from different life stages in <60 s. We successfully detected DNA from adult worms, eggs and infected snails. The speed and simplicity of this method enables the point-of-care detection of S. japonicum.
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